例句與造句

1. Reverse transcription , rt
   用反轉(zhuǎn)錄
2. Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus
   傳染性造血器官壞死病毒逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)操作規(guī)程
3. Influenza virus sample in cultured cells was treated for reverse transcription , amplification , fluorescent dye labelling , hybridization and scanning
   對(duì)待檢樣品進(jìn)行隨機(jī)逆轉(zhuǎn)錄和擴(kuò)增，熒光標(biāo)記，雜交，掃描分析。
4. Pgem - t vector system , jm109 competent cells , reverse transcription kit and in vitro translation kit were purchased from promega company
   Pgem一t載體，感受態(tài)細(xì)菌jm109 ，反轉(zhuǎn)錄試劑盒和體外翻譯試劑盒均購(gòu)自promega公司。
5. The ghr cdna was isolated from southern catfish ( silurus meridionalis ) by reverse transcription polymerase chain reaction followed by rapid amplification of cdna ends
   用rt - per加race的方法從南方大口鲇中克隆出其生長(zhǎng)激素受體( ghr ) 。
6. It's difficult to find reverse transcription in a sentence. 用reverse transcription造句挺難的
7. The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119 , gxbm were amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) , respectively
   對(duì)三株的n基因和119 、 gxbm株的g基因進(jìn)行了rt - pcr擴(kuò)增、克隆和測(cè)序。
8. Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna
   采用cartesian基因芯片打印儀，將純化的cdna片段打印在corning玻片表面，制作了酵母細(xì)胞基因表達(dá)譜芯片。
9. First , mrna was purified from the total rna extracted from fresh spleens of nonimmunized mice of kunming , then the cdna library was achieved via reverse transcription pcr . gene fragments encoding v
   首先從未免疫小鼠的脾臟中提取總rna ，分離出mrna ，經(jīng)逆轉(zhuǎn)錄合成其總cdna 。
10. The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) method using isolated total rna as template
    應(yīng)用逆轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)( rt - pcr )技術(shù)克隆得到編碼草魚生長(zhǎng)激素( cgh )的基因cdna ，并定向克隆到puc18載體上。
11. Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
    用qiagen的rneasyrna試劑盒提取病毒rna后，逆助興才李醫(yī)‘筍脂碩圣‘老‘才轉(zhuǎn)錄合成cdna 。
12. After one - step reverse transcription of the rna virus genomes , the viruses " specific dna fragments were amplified by nested three loci pcr , which the amplification system was optimized by uniform design
    選擇合適的核酸抽提方法，一步法抽提hbv 、 hcv及hiv 1的病毒核酸并一步法逆轉(zhuǎn)錄病毒的rna ，獲得病毒的dna 。
13. Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits . after reverse transcription , cdna was amplified using specific primers for each gene segment
    用qiagen的rneasyrna提取試劑盒按說(shuō)明提取，提取的rna逆轉(zhuǎn)錄合成cdna ， cdna為pgr反應(yīng)擴(kuò)增的模板，特異性的引物是針對(duì)各個(gè)基因片段設(shè)計(jì)的。
14. A about 1 . 0kb dna fragment was amplified by reverse transcription polymerase chain reaction ( rt - pcr ) . the dna fragment was then successfully cloned into pgem - t - easy vector , which was confirmed by sequence analysis and restrict enzyme identification
    將此基因克隆到pgem - t - easy載體中，經(jīng)pcr和測(cè)序鑒定，成功地克隆了a毒株的ha1基因。
15. Comparing with using mulv reverse transcriptase or amv reverse transcriptase to do reverse transcription at 3 7 - 42 " c , more accurate and complete dna could be gotten by using tag dna polymerase at 72 c since dimeric structure of mrna was opened fully at high temperature
    與在37 - 42用mulv或amv進(jìn)行反轉(zhuǎn)錄相比，在較高溫度下( 72 )用taqdna聚合酶進(jìn)行反轉(zhuǎn)錄有利于正確、完整的cdna ，因?yàn)樵诟邷叵耺rna的二級(jí)結(jié)構(gòu)被充分打開(kāi)。
16. Total rna was extracted from the second stage larve of hypoderma sp , then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer . the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software
    本試驗(yàn)的目的旨在進(jìn)行hypodeminc ( hc )和hypodermina ( ha )基因的克隆、測(cè)序、構(gòu)建重組表達(dá)載體并誘導(dǎo)表達(dá)，獲得重組抗原，以解決天然抗原的不足并為診斷和免疫試劑的產(chǎn)業(yè)化奠定基礎(chǔ)。
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